Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization

KIZ OpenIR > 生殖与发育生物学

	Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization
	Si W 1,3; Zheng P 1,3; Tang XH 1,3; He XC 1,3; Wang H 1,3; Bavister BD 2,3; Ji WZ*1,3; wji@mail.kiz.ac.cn
	2000
发表期刊	CRYOBIOLOGY
ISSN	0011-2240
卷号	41 期号:3 页码:232-240
合作性质	其它
摘要	Although spermatozoa from several species of nonhuman primates have been cryopreserved, there has been no report of success with rhesus macaque spermatozoa as judged by functional assays. Two Tris--egg yolk freezing media, TEST and TTE, which have been successfully used for cynomolgus macaque (Macaca fascicularis) spermatozoa, were compared for cryopreservation of spermatozoa from four rhesus macaques (Macaca mulatta). The postthaw motility (percentage and duration) of spermatozoa cryopreserved in TTE was much higher than that for spermatozoa cryopreserved in TEST. The function of sperm cryopreserved in TTE was evaluated by in vitro fertilization of oocytes collected from gonadotropin-stimulated prepubertal rhesus macaques. Of the inseminated oocytes, 82 +/- 13% were fertilized and 63 +/- 22 and 39 +/- 21% of the resulting zygotes developed into morulae and blastocysts, respectively. These results indicate that rhesus macaque spermatozoa can be effectively cryopreserved in TTE medium. This finding will facilitate the application of in vivo and in vitro assisted reproductive technologies in this species.
关键词	Cryopreservation Macaca Mulatta Spermatozoa Cryoprotectants n Vitro Fertilization Embryo Development Sugar Glycerol Semen Capacitation Plasma Membrane
资助者	This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840). ; This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840). ; This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840). ; This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840).
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收录类别	SCI
语种	英语
资助者	This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840). ; This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840). ; This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840). ; This work was supported by grants from the China Na- tional Science Foundation (39970117), the Chinese Acad- emy of Sciences (KZ952-J1-109), the Yunnan Nature Sci- ences Foundation (98C092M), and the Fogarty International Center, National Institutes of Health, U.S.A. (TW00840).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5535
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, The Chinese Academy of Science, Kunming, Yunnan 650223, People’s Republic of China 2.Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin 53706, U.S.A. 3.The China–U.S. Primate Biology Laboratory, Kunming Institute of Zoology and University of Wisconsin-Madison
推荐引用方式 GB/T 7714	Si W,Zheng P,Tang XH,et al. Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization[J]. CRYOBIOLOGY,2000,41(3):232-240.
APA	Si W.,Zheng P.,Tang XH.,He XC.,Wang H.,...&wji@mail.kiz.ac.cn.(2000).Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization.CRYOBIOLOGY,41(3),232-240.
MLA	Si W,et al."Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization".CRYOBIOLOGY 41.3(2000):232-240.

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