Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm

KIZ OpenIR > 生殖与发育生物学

	Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm
	Si W 1,2; Zheng P 1; Li YH 1,3; Dinnyes A 4,5; Ji WZ*1; wji@mail.kiz.ac.cn
	2004
发表期刊	AMERICAN JOURNAL OF PRIMATOLOGY
ISSN	0275-2565
卷号	62 期号:4 页码:301-306
合作性质	其它
摘要	Glycerol and dimethyl sulfoxide (DMSO) are widely used as penetrating cryoprotectants in the freezing of sperm, and various concentrations are applied in different species and laboratories. The present study aimed to examine the effect of these two cryoprotectants at different concentrations (2%, 5%, 10%, and 15% glycerol or DMSO) on rhesus monkey sperm cryopreservation. The results showed that the highest recovery of post-thaw sperm motility, and plasma membrane and acrosome integrity was achieved when the sperm was frozen with 5% glycerol. Spermatozoa cryopreserved with 15% DMSO showed the lowest post-thaw sperm motility, and spermatozoa cryopreserved with 15% glycerol and 15% DMSO showed the lowest plasma membrane integrity among the eight groups. The results achieved with 5% glycerol were significantly better for all parameters than those obtained with 5% DMSO. The functional cryosurvival of sperm frozen with 5% glycerol was further assessed by in vitro fertilization (IVF). Overall, 85.7% of the oocytes were successfully fertilized, and 51.4% and 5.7% of the resulting zygotes developed into morulae and blastocysts, respectively. The results indicate that the type and concentration of the penetrating cryoprotectant used can greatly affect the survival of rhesus monkey sperm after it is frozen and thawed. The suitable glycerol level for rhesus monkey sperm freezing is 5%, and DMSO is not suitable for rhesus monkey sperm cryopreservation.
关键词	Cryopreservation Rhesus Monkey Spermatozoa Glycerol Dmso
资助者	Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02.
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收录类别	SCI
语种	英语
资助者	Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009–09; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Techno- logical Collaboration Project; Contract grant number: TET CHN14/02.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5573
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, Beijing, People’s Republic of China 3.College of Food Science and Technology, Yunnan Agricultural University, Kunming, People’s Republic of China 4.Research Group for Applied Animal Genetics and Biotechnology, Hungarian Academy of Sciences and Szent Istvan University, Godollo, Hungary 5.Department of Animal Biology, Agricultural Biotechnology Center, Godollo, Hungary
推荐引用方式 GB/T 7714	Si W,Zheng P,Li YH,et al. Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm[J]. AMERICAN JOURNAL OF PRIMATOLOGY,2004,62(4):301-306.
APA	Si W,Zheng P,Li YH,Dinnyes A,Ji WZ*,&wji@mail.kiz.ac.cn.(2004).Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm.AMERICAN JOURNAL OF PRIMATOLOGY,62(4),301-306.
MLA	Si W,et al."Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm".AMERICAN JOURNAL OF PRIMATOLOGY 62.4(2004):301-306.

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