A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm

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	A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm
	Su L*1,2; Li XL 1; Quan JX 3; Yang SH 1; Li YH 1; He XC 1; Tang XH 1; suleil@hotmail.com
	2008
发表期刊	ANIMAL REPRODUCTION SCIENCE
ISSN	0378-4320
卷号	104 期号:2-4 页码:212-219
合作性质	其它
摘要	Cryopreservation of domestic animal sperm has been widely used for artificial insemination (AI), and egg yolk is one of the most commonly used cryoprotectants during the freezing–thawing process. The objectives of this study were to compare the effectiveness of egg yolk from five avian species (domestic chicken, domestic duck, domestic goose, Japanese quail or domestic pigeon) and to optimize the concentration of egg yolk on the cryopreservation of bull sperm in terms of frozen–thawed sperm progressive motility and viability.Theresultsweretwo-fold.First,theyshowedthatpigeoneggyolkprovidedthebestcryoprotective effects on the cryopreservation of bull sperm, compared with egg yolk of chicken, quail, goose or duck. Second, the best concentration of pigeon egg yolk in extender was 20% during cryopreservation among five concentrations of 5, 10, 20, 30 or 40%. The results suggest that pigeon egg yolk could be used as analternative to chicken egg yolk in extender but requires further testing in fertility trials.
关键词	Cryopreservation Bull Sperm Egg Yolk Sperm Progressive Motility Viability
资助者	This study was sponsored by Grant of “Western Light” and “Keji Zhiqian” from Chinese AcademyofScience;NDRC,MinistryofScienceandTechnologyofPeople’sRepublicofChina; Yunnan State Grant of Science and Technology Projects, awarded to Dr. Su. ; This study was sponsored by Grant of “Western Light” and “Keji Zhiqian” from Chinese AcademyofScience;NDRC,MinistryofScienceandTechnologyofPeople’sRepublicofChina; Yunnan State Grant of Science and Technology Projects, awarded to Dr. Su. ; This study was sponsored by Grant of “Western Light” and “Keji Zhiqian” from Chinese AcademyofScience;NDRC,MinistryofScienceandTechnologyofPeople’sRepublicofChina; Yunnan State Grant of Science and Technology Projects, awarded to Dr. Su. ; This study was sponsored by Grant of “Western Light” and “Keji Zhiqian” from Chinese AcademyofScience;NDRC,MinistryofScienceandTechnologyofPeople’sRepublicofChina; Yunnan State Grant of Science and Technology Projects, awarded to Dr. Su.
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收录类别	SCI
语种	英语
资助者	This study was sponsored by Grant of “Western Light” and “Keji Zhiqian” from Chinese AcademyofScience;NDRC,MinistryofScienceandTechnologyofPeople’sRepublicofChina; Yunnan State Grant of Science and Technology Projects, awarded to Dr. Su. ; This study was sponsored by Grant of “Western Light” and “Keji Zhiqian” from Chinese AcademyofScience;NDRC,MinistryofScienceandTechnologyofPeople’sRepublicofChina; Yunnan State Grant of Science and Technology Projects, awarded to Dr. Su. ; This study was sponsored by Grant of “Western Light” and “Keji Zhiqian” from Chinese AcademyofScience;NDRC,MinistryofScienceandTechnologyofPeople’sRepublicofChina; Yunnan State Grant of Science and Technology Projects, awarded to Dr. Su. ; This study was sponsored by Grant of “Western Light” and “Keji Zhiqian” from Chinese AcademyofScience;NDRC,MinistryofScienceandTechnologyofPeople’sRepublicofChina; Yunnan State Grant of Science and Technology Projects, awarded to Dr. Su.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5631
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）
通讯作者	suleil@hotmail.com
作者单位	1.Kunming Institute of Zoology, Chinese Academy of Science, Kunming, Yunnan 650223 PR China 2.Center of Yunnan Zhongke Embryo Biotechnology, Kunming, Yunnan 650217 PR China 3.Bureau of Qingdao Entry-Exit Inspection and Quarantine, Qingdao, Shangdong 266002 PR China
推荐引用方式 GB/T 7714	Su L*,Li XL,Quan JX,et al. A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm[J]. ANIMAL REPRODUCTION SCIENCE,2008,104(2-4):212-219.
APA	Su L*.,Li XL.,Quan JX.,Yang SH.,Li YH.,...&suleil@hotmail.com.(2008).A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm.ANIMAL REPRODUCTION SCIENCE,104(2-4),212-219.
MLA	Su L*,et al."A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm".ANIMAL REPRODUCTION SCIENCE 104.2-4(2008):212-219.

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