DNA sequence analysis of the triose phosphate isomerase gene from isolates of Giardia lamblia

	DNA sequence analysis of the triose phosphate isomerase gene from isolates of Giardia lamblia
其他题名	蓝氏贾第虫分离株tim基因序列分析比较研究
	Lu SQ*1; Wen JF 2; Li JH 1; Wang FY 1; lusiqi@cpums.edu.cn
	2002
发表期刊	CHINESE MEDICAL JOURNAL
ISSN	0366-6999
卷号	115 期号:1 页码:99-102
合作性质	其它
摘要	Objective To confirm the genetic relation between Giardia lamblia (G. lamblia) isolates from different geographic regions of China and other countries. Methods Genomic DNA were extracted from the trophozoites or cysts of Giardia lamblia. The triose phosphate isomerase (tim) gene was amplified using polymerase chain reaction (PCR) technique. PCR products were digested with endonuclease and sequenced. The data of sequencing were analyzed with the DNAstar software and compared with that of the isolates acquired from GenBank. Results Of nine isolates of Giardia lamblia from China (C1, C2, CH2 and CH3), Cambodia (CAM), Australia (A1 and A2) and America (BP and CDC), respectively, 3 (A1, A2 and CAM) fit into Group 1 (WB), 2 (CH2 and CH3) into Group 2, and 4 (C1, C2, BP and CDC) into Group 3 (GS). The results confirmed the genetic relatedness of G. lamblia isolates from all over the world. Conclusion Genotyping isolates of G. Lamblia provides important information for establishing the phylogenetic relationship or for the epidemiological evaluation of the spreading of this organism.
其他摘要	目的：为了探讨来源于中国和其它国家不同地理位置贾第虫分离株之间的遗传学关系。方法：从贾第虫滋养体和包囊提取总DNA。对磷酸丙糖异构酶基因进行PCR扩增。PCR产物经限制性内切酶消化、序列测定及分析。对所得DNA序列数据用DNAstar软件处理并与登录基因库虫株的序列比较。结果：在来自我国（C1、C2、CH2、CH3），柬埔寨（CAM）、澳大利亚（A1、A2）和美国（BP、CDC）的9株蓝氏贾第虫中，A1、A2和CAM属于第1型（WB）；CH2和CH3属于第2型（JH）；C1、C2、BP和CDC属于第3型（GS）。结论：贾第虫分离株基因型的确定可分为本虫分子系统进货和分子流行病学研究提供重要资料。
关键词	Giardia Lamblia Tim Gene Gene Sequence Analysis Genotype
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收录类别	其他
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5951
专题	科研部门_真核细胞进化基因组（文建凡）细胞与分子进化开放实验室
通讯作者	lusiqi@cpums.edu.cn
作者单位	1.Capital Univ Med Sci, Dept Cell Biol, Beijing 100054, Peoples R China 2.Chinese Acad Sci, Kunming Inst Zool, Lab Cellular & Mol Evolut, Kunming 650223, Peoples R China
推荐引用方式 GB/T 7714	Lu SQ*,Wen JF,Li JH,et al. DNA sequence analysis of the triose phosphate isomerase gene from isolates of Giardia lamblia[J]. CHINESE MEDICAL JOURNAL,2002,115(1):99-102.
APA	Lu SQ*,Wen JF,Li JH,Wang FY,&lusiqi@cpums.edu.cn.(2002).DNA sequence analysis of the triose phosphate isomerase gene from isolates of Giardia lamblia.CHINESE MEDICAL JOURNAL,115(1),99-102.
MLA	Lu SQ*,et al."DNA sequence analysis of the triose phosphate isomerase gene from isolates of Giardia lamblia".CHINESE MEDICAL JOURNAL 115.1(2002):99-102.

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