Construction, characterization and FISH mapping of a bacterial artificial chromosome library of Chinese pangolin (Manis pentadactyla)

	Construction, characterization and FISH mapping of a bacterial artificial chromosome library of Chinese pangolin (Manis pentadactyla)
	Che J 1,3; Wang J 1; Su W 1; Ye J 1,3; Wang Y 1; Nie W 1; Yang F*1,2; fy1@sanger.ac.uk
	2008
发表期刊	CYTOGENETIC AND GENOME RESEARCH
卷号	122 期号:1 页码:55–60
合作性质	其它
摘要	Chinese pangolins as a representative species in the order Pholidota have highly specified morphological characters and occupy an important place in the mammalian phylogenetic tree. To obtain genomic data for this species, we have constructed a bacterial artificial chromosome (BAC) library of Chinese pangolin. The library contains 208,272 clones with an average insert size of 122.1 kb and represents approximately eight times the Chinese pangolin haploid genome (if we assume that the Chinese pangolins have a genome size similar to human). One hundred and twenty randomly-selected BAC clones were mapped onto Chinese pangolin chromosomes by fluorescence in situ hybridization (FISH), showing a largely unbiased chromosomal distribution. Several clones containing repetitive DNA and ribosomal DNA genes were also found. The BAC library and FISH mapped BAC clones are useful resources for comparative genomics and cytogenetics of mammals and in particular, the ongoing genome sequencing project of Chinese pangolins. Copyright (C) 2008 S. Karger AG, Basel.
资助者	This study is partly supported by a grant from the National Natural Science Foundation of China (No. 30270719). ; This study is partly supported by a grant from the National Natural Science Foundation of China (No. 30270719). ; This study is partly supported by a grant from the National Natural Science Foundation of China (No. 30270719). ; This study is partly supported by a grant from the National Natural Science Foundation of China (No. 30270719).
收录类别	SCI
语种	英语
资助者	This study is partly supported by a grant from the National Natural Science Foundation of China (No. 30270719). ; This study is partly supported by a grant from the National Natural Science Foundation of China (No. 30270719). ; This study is partly supported by a grant from the National Natural Science Foundation of China (No. 30270719). ; This study is partly supported by a grant from the National Natural Science Foundation of China (No. 30270719).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6377
专题	科研部门_两栖爬行类多样性与进化（车静）遗传资源与进化国家重点实验室遗传资源与进化国家重点实验室_保护遗传学(细胞库)
通讯作者	fy1@sanger.ac.uk
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming (P.R. China) 2.Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge (UK) 3.The Graduate School of the Chinese Academy of Sciences, Beijing (P.R. China )
推荐引用方式 GB/T 7714	Che J,Wang J,Su W,et al. Construction, characterization and FISH mapping of a bacterial artificial chromosome library of Chinese pangolin (Manis pentadactyla)[J]. CYTOGENETIC AND GENOME RESEARCH,2008,122(1):55–60.
APA	Che J.,Wang J.,Su W.,Ye J.,Wang Y.,...&fy1@sanger.ac.uk.(2008).Construction, characterization and FISH mapping of a bacterial artificial chromosome library of Chinese pangolin (Manis pentadactyla).CYTOGENETIC AND GENOME RESEARCH,122(1),55–60.
MLA	Che J,et al."Construction, characterization and FISH mapping of a bacterial artificial chromosome library of Chinese pangolin (Manis pentadactyla)".CYTOGENETIC AND GENOME RESEARCH 122.1(2008):55–60.

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