Apoptotic activity of frog Bombina maxima skin albumin

	Apoptotic activity of frog Bombina maxima skin albumin
	Zhang YX 1,3; Wang YY 2,3; Lee WH 1; Zheng YT 2; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2006
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY,Part B
卷号	143 期号:2 页码:153-159
合作性质	其它
摘要	Albumin, the most abundant protein components of blood plasma, is synthesized and secreted by liver cells in vertebrates. Recently, it was demonstrated that frog Bombina maxima albumin is also expressed in skin. Both B. maxima albumins from skin and serum (BmA-skin and BmA-serum) have similar biochemical characteristics except that the former contains haem b. Present studies showed that BmA-skin exhibited cytotoxic activity on H9 and C8166 cells. Pretreated with hemin to induce erythroid differentiation, K562 cells lost their resistance to cytotoxicity of BmA-skin. After treating cells with BmA-skin for 48 h, 50 percentage cytotoxic concentrations (CC(50)) of BmA-skin on H9, C8166 and hemin-treated K562 cells were 1.31+/-0.09, 1.59+/-0.08 and 2.28+/-0.06 microM, respectively. The cell death induced by BmA-skin was mediated by apoptosis of the tested cell lines, as demonstrated by nuclear morphological changes, DNA fragmentation and DNA hypodiploidy of apoptosis cells. At BmA-skin concentration of 2 microM, 27.3%, 19.7% and 17.8% of H9, C8166 and hemin-treated K562 cells were found to be apoptotic. In contrast, BmA-serum possessed no cytotoxic and apoptosis-inducing activity on all the cell lines tested, even with concentration used up to 15 microM. These results indicated that bound haem b in BmA-skin contributed significantly to its cytotoxic and apoptosis-inducing activity on the cell lines assayed.
关键词	Albumin Amphibian Apoptosis Frog Haem Skin Serum Cytotoxicity
资助者	This work was supported by the grants of “Western Light” ProjectsfromtheChineseAcademyofSciencestoDrs.Y.Zhang and W.H. Lee, and grants from National Natural Science Foun- dation (30570359, 30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M2005PY01-23). ; This work was supported by the grants of “Western Light” ProjectsfromtheChineseAcademyofSciencestoDrs.Y.Zhang and W.H. Lee, and grants from National Natural Science Foun- dation (30570359, 30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M2005PY01-23). ; This work was supported by the grants of “Western Light” ProjectsfromtheChineseAcademyofSciencestoDrs.Y.Zhang and W.H. Lee, and grants from National Natural Science Foun- dation (30570359, 30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M2005PY01-23). ; This work was supported by the grants of “Western Light” ProjectsfromtheChineseAcademyofSciencestoDrs.Y.Zhang and W.H. Lee, and grants from National Natural Science Foun- dation (30570359, 30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M2005PY01-23).
收录类别	其他
语种	英语
资助者	This work was supported by the grants of “Western Light” ProjectsfromtheChineseAcademyofSciencestoDrs.Y.Zhang and W.H. Lee, and grants from National Natural Science Foun- dation (30570359, 30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M2005PY01-23). ; This work was supported by the grants of “Western Light” ProjectsfromtheChineseAcademyofSciencestoDrs.Y.Zhang and W.H. Lee, and grants from National Natural Science Foun- dation (30570359, 30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M2005PY01-23). ; This work was supported by the grants of “Western Light” ProjectsfromtheChineseAcademyofSciencestoDrs.Y.Zhang and W.H. Lee, and grants from National Natural Science Foun- dation (30570359, 30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M2005PY01-23). ; This work was supported by the grants of “Western Light” ProjectsfromtheChineseAcademyofSciencestoDrs.Y.Zhang and W.H. Lee, and grants from National Natural Science Foun- dation (30570359, 30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M2005PY01-23).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6414
专题	科研部门_分子免疫药理学（郑永唐）科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Zhang YX,Wang YY,Lee WH,et al. Apoptotic activity of frog Bombina maxima skin albumin[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY,Part B,2006,143(2):153-159.
APA	Zhang YX,Wang YY,Lee WH,Zheng YT,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2006).Apoptotic activity of frog Bombina maxima skin albumin.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY,Part B,143(2),153-159.
MLA	Zhang YX,et al."Apoptotic activity of frog Bombina maxima skin albumin".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY,Part B 143.2(2006):153-159.

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