| Apparent mtDNA sequence heterogeneity in single human blood CD34+ cells is markedly affected by storage and transport | |
| Yao YG*1,2; Kajigaya S2; Samsel L2; McCoy JP2; Torelli G3; Young NS2; ygyaozh@gmail.com | |
| 2013 | |
| 发表期刊 | MUTATION RESEARCH
 |
| 卷号 | 751-572期号:X页码:36– 41 |
| 合作性质 | 其它 |
| 摘要 | Single CD34(+) cells from adult human peripheral blood show mtDNA sequence heterogeneity. In this study, we compared mtDNA sequence variation in single CD34(+) cells from peripheral blood (PB) mononuclear cells (MNCs) from the same donors but under different conditions of storage and transport: group I, MNCs from heparinized PB that inadvertently required six days to be transported to the testing laboratory; group II, MNCs which were isolated from PB within a day of phlebotomy and frozen prior to transportation and storage. We observed more cell death for MNCs of group I than group II. Concordantly, group I CD34(+) cells had a very low potential for hematopoietic colony formation in vitro compared with group II cells. CD34(+) cells of group II showed an unexpectedly higher level of mtDNA sequence heterogeneity than was present in group I cells. These observations suggest that reduced mtDNA sequence heterogeneity in single CD34(+) cells of group I was likely due to elimination of cells harboring mutations. CD34+ cells that survive stress ex vivo may be more enriched in quiescent primitive hematopoietic stem cells, with fewer mtDNA mutations than are present in committed progenitors. Technically, attention is required to conditions of preparation of human blood samples for single cell mtDNA analysis |
| 关键词 | Mtdna Single Cell Analysis Mutation Hematopoietic Stem Cell Committed Progenitors |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/7736 |
| 专题 | 科研部门_疾病机理遗传学和进化医学学科组（姚永刚） 科研部门_动物模型与人类重大疾病机理重点实验室 |
| 通讯作者 | ygyaozh@gmail.com |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, Chin 2.Hematology Branch and Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA 3.Dipartimento di Oncologia ed Ematologia, Università di Modena e Reggio Emilia, Policlinico – Via del Pozzo 71, Modena 41100, Italy |
| 推荐引用方式 GB/T 7714 | Yao YG*,Kajigaya S,Samsel L,et al. Apparent mtDNA sequence heterogeneity in single human blood CD34+ cells is markedly affected by storage and transport[J]. MUTATION RESEARCH,2013,751-572(X):36– 41. |
| APA | Yao YG*.,Kajigaya S.,Samsel L.,McCoy JP.,Torelli G.,...&ygyaozh@gmail.com.(2013).Apparent mtDNA sequence heterogeneity in single human blood CD34+ cells is markedly affected by storage and transport.MUTATION RESEARCH,751-572(X),36– 41. |
| MLA | Yao YG*,et al."Apparent mtDNA sequence heterogeneity in single human blood CD34+ cells is markedly affected by storage and transport".MUTATION RESEARCH 751-572.X(2013):36– 41. |
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| yao13-01.pdf（1251KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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