Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing

	Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing
	Zhang Y1,2 ; Yu GY 1,2,3; Lee WH 1; Zhang Y*1; Xiang Y 1,2; Wu JB 1; Jiang P 1,2; zhangy@mail.kiz.ac.cn
	2010
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷号	398 期号:3 页码:559-564
摘要	Toad skin is naked and continually confronted by various injurious factors. Constant skin renewal and repairs occur frequently. However, the mechanisms of the renewal and repair have not clearly elucidated. In our previous work, a trefoil factor (TFF), Bm-TFF2, has been purified from the Bombina maxima skin and characterized as a platelet agonist. The mRNA of TFFs in toad skin was up-regulated greatly during the metamorphosis, indicating a pivotal role of TFFs in amphibian skin. Here, we presented the effects of Bm-TFF2 on the cell migration, apoptosis and proliferation. Bm-TFF2 bound to epithelial cells and showed strong cell motility activity. At the concentrations of 1-100 nM, Bm-TFF2-induced migration of human epithelial AGS and HT-29 cells, and rat intestinal epithelial IEC-6 cell lines. The in vitro wound healing assay also verified the activity of Bm-TFF2. Bm-TFF2 could also inhibit cell apoptosis induced by ceramide and sodium butyrate. The cell migration-promoting activity was abolished by MEK1 inhibitors, U0126 and PD98059, suggesting that ERK1/2 activation is crucial for Bm-TFF2 to stimulate cell migration. Taken together, Bm-TFF2 promoted wound healing by stimulating cell migration via MAPK pathway and preventing cell apoptosis. The potent biological activity of Bm-TFF2 makes it a useful molecular tool for further studies of structure-function relationship of the related human TFFs.
关键词	Amphibian Skin Trefoil Factor Cell Migration Wound Healing Anti-apoptosis
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/9606
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of Chinese Academy of Sciences, Beijing 100049, China 3.Department of Biochemistry, Kunming Medical College, Kunming, Yunnan 650032, China
推荐引用方式 GB/T 7714	Zhang Y,Yu GY,Lee WH,et al. Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,398(3):559-564.
APA	Zhang Y.,Yu GY.,Lee WH.,Zhang Y*.,Xiang Y.,...&zhangy@mail.kiz.ac.cn.(2010).Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,398(3),559-564.
MLA	Zhang Y,et al."Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 398.3(2010):559-564.

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