Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm

KIZ OpenIR > 生殖与发育生物学

	Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm
	Yang SH 1,2; Ping SH 1,2; Si W[*]2; He XC 2; Wang XY 2; Lu YQ 2; Ji SH 2; Niu YY 2; Ji WZ 2,3; siw@mail.kiz.ac.cn
	2011
发表期刊	JOURNAL OF VETERINARY MEDICAL SCIENCE
卷号	73 期号:6 页码:717-723
合作性质	其它
摘要	Ethylene glycol (EG) has been speculated to be the most appropriate penetrating cryoprotectant for cryopreservation of rhesus macaque sperm due to its higher permeability coefficient. The present study aimed to determine the optimal EG concentration, freezing rate and holding time in liquid nitrogen (LN(2)) vapor for rhesus sperm cryopreservation. Among six tested EG concentrations (0, 0.18, 0.35, 0.7, 1.4 and 2.1 M), 0.7 M EG showed the most effective cryoprotection (P<0.05). Sperm frozen with 0.7 M EG at -183 degrees C/min showed higher post-thaw motility than sperm frozen at -10, -67 or -435 degrees C/min (P<0.05). Sperm frozen in LN(2) vapor at -183 degrees C/min with 0.7 M EG and a holding time of 10 min showed higher post-thaw motility compared with a holding time of 5 or 15 min (P<0.05). The function of sperm cryopreserved at the optimized EG concentration, freezing rate and holding time was further evaluated by in vitro fertilization. Of the 36 oocytes collected from gonadotropin-stimulated rhesus macaques, 61.1% were fertilized, and 61.1, 44.4 and 36.1% of the oocytes developed to 2 cells, morulae and blastocysts, respectively. Our findings provide an alternative penetrating cryoprotectant and optimal protocol for genetic preservation purposes in this important species.
关键词	Cryopreservation Freezing Of Semen In Vitro Fertilization Rhesus Macaque
资助者	This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232). ; This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232). ; This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232). ; This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232).
收录类别	SCI
语种	英语
资助者	This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232). ; This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232). ; This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232). ; This work was supported by research grants from the National Key Technologies R & D Program of China (2007BAI33B00), the Chinese Major State Basic Development Program (2006CB701505 and 2007CB947701) and the Chinese National Science Founda- tion (31071279 and 30871232).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6630
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）中国科学院昆明灵长类研究中心
通讯作者	siw@mail.kiz.ac.cn
作者单位	1.Laboratory of Moleculac Genetics of Aging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224 2.Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming 650223 3.Kunming Biomed International, National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, P.R. China
推荐引用方式 GB/T 7714	Yang SH,Ping SH,Si W[*],et al. Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm[J]. JOURNAL OF VETERINARY MEDICAL SCIENCE,2011,73(6):717-723.
APA	Yang SH.,Ping SH.,Si W[*].,He XC.,Wang XY.,...&siw@mail.kiz.ac.cn.(2011).Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm.JOURNAL OF VETERINARY MEDICAL SCIENCE,73(6),717-723.
MLA	Yang SH,et al."Optimization of Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Cryopreservation of Rhesus Macaque (Macaca mulatta) Sperm".JOURNAL OF VETERINARY MEDICAL SCIENCE 73.6(2011):717-723.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2011080903.pdf（1098KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文