Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami

	Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami
	Chen WL 1,4; Yang XN 3; Chen LL 1,5; Yang XL 1; Feng FF 1; He WY 1; Yu HN1,2; Liu JZ1; jzliu21@heinfo.net ; joannyu@live.cn
	2011
发表期刊	BIOCHIMIE
卷号	93 期号:7 页码:1110-1114
合作性质	其它
摘要	Amphibian opiate peptides including dermorphins and deltorpins have been recently found only in the skin of South American frogs belonging to the subfamily Phyllomedusinae (Phyllomedusa, Agalychnis and Pachymedusa species). No opiate peptides have ever been identified from other amphibians or organs except skin. Here we report the purification and characterization of a novel antinociceptive peptide named odorranaopin from the homogenates of the frog brains, Odorrana grahami, which is also the first antinociceptive peptide found in Ranidae amphibian. Odorranaopin comprises 17 amino acid residues with the sequence of DYTIRTRLHQESSRKVL (Mr 2102 Da). The cDNA encoding odorranaopin was cloned from the frog brain cDNA library, and it was confirmed to be a specific gene. The odorranaopin precursor deduced is composed of 61 amino acid residues including the predicted signal peptide, acidic spacer peptide and mature odorranaopin positioned at the C-terminus. Odorranaopin could inhibit nociceptive responses induced by formalin and acetic acid. It also inhibited the contractile responses of ileum smooth muscle induced by bradykinin, implying that the antinociceptive activity of odorranaopin possibly results from its blockade on bradykinin or bradykinin receptor functions. Odorranaopin is the first antinociceptive peptide found in Ranidae amphibian.
关键词	Amphibian Nociceptive Opiate Peptide Odorrana Grahami Defense
资助者	This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098). ; This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098). ; This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098). ; This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098).
收录类别	SCI
语种	英语
资助者	This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098). ; This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098). ; This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098). ; This work was supported by the grants from Chinese National Natural Science Foundation (30900240, 41076098).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6637
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	jzliu21@heinfo.net; joannyu@live.cn
作者单位	1.College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China 2.School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China 3.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 4.Yunnan Clinical Research Center of Breast Cancer, The Third Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China 5.Clinical Laboratory, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China
推荐引用方式 GB/T 7714	Chen WL,Yang XN,Chen LL,et al. Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami[J]. BIOCHIMIE,2011,93(7):1110-1114.
APA	Chen WL.,Yang XN.,Chen LL.,Yang XL.,Feng FF.,...&joannyu@live.cn.(2011).Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami.BIOCHIMIE,93(7),1110-1114.
MLA	Chen WL,et al."Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami".BIOCHIMIE 93.7(2011):1110-1114.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2011080910.pdf（514KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文