A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus

	A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus
	Seipke RF1; Barke J 1; Brearley C 1; Hill L 2; Yu DW 1,3; Goss RJM 4; Hutchings MI1; r.seipke@uea.ac.uk ; m.hutchings@uea.ac.uk
	2011
发表期刊	PLOS ONE
卷号	6 期号:8 页码:e22028
合作性质	其它
摘要	Attine ants are dependent on a cultivated fungus for food and use antibiotics produced by symbiotic Actinobacteria as weedkillers in their fungus gardens. Actinobacterial species belonging to the genera Pseudonocardia, Streptomyces and Amycolatopsis have been isolated from attine ant nests and shown to confer protection against a range of microfungal weeds. In previous work on the higher attine Acromyrmex octospinosus we isolated a Streptomyces strain that produces candicidin, consistent with another report that attine ants use Streptomyces-produced candicidin in their fungiculture. Here we report the genome analysis of this Streptomyces strain and identify multiple antibiotic biosynthetic pathways. We demonstrate, using gene disruptions and mass spectrometry, that this single strain has the capacity to make candicidin and multiple antimycin compounds. Although antimycins have been known for > 60 years we report the sequence of the biosynthetic gene cluster for the first time. Crucially, disrupting the candicidin and antimycin gene clusters in the same strain had no effect on bioactivity against a co-evolved nest pathogen called Escovopsis that has been identified in similar to 30% of attine ant nests. Since the Streptomyces strain has strong bioactivity against Escovopsis we conclude that it must make additional antifungal(s) to inhibit Escovopsis. However, candicidin and antimycins likely offer protection against other microfungal weeds that infect the attine fungal gardens. Thus, we propose that the selection of this biosynthetically prolific strain from the natural environment provides A. octospinosus with broad spectrum activity against Escovopsis and other microfungal weeds.
资助者	This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY. ; This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY. ; This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY. ; This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY.
收录类别	SCI
语种	英语
资助者	This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY. ; This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY. ; This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY. ; This work was funded by the Medical Research Council Milstein Award (grant G0801721) to MIH, RJMG and DWY.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6752
专题	科研部门_生态学与环境保护中心（俞维理）遗传资源与进化国家重点实验室
通讯作者	r.seipke@uea.ac.uk; m.hutchings@uea.ac.uk
作者单位	1.School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United Kingdom 2.Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom 3.State Key Laboratory of Genetic Resources, and Evolution, Ecology, Conservation, and Environment Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 4.School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, United Kingdom
推荐引用方式 GB/T 7714	Seipke RF*,Barke J,Brearley C,et al. A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus[J]. PLOS ONE,2011,6(8):e22028.
APA	Seipke RF*.,Barke J.,Brearley C.,Hill L.,Yu DW.,...&m.hutchings@uea.ac.uk.(2011).A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus.PLOS ONE,6(8),e22028.
MLA	Seipke RF*,et al."A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus".PLOS ONE 6.8(2011):e22028.

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