Hepatocytic differentiation of rhesus monkey embryonic stem cells promoted by collagen gels and growth factors

KIZ OpenIR > 生殖与发育生物学

	Hepatocytic differentiation of rhesus monkey embryonic stem cells promoted by collagen gels and growth factors
	Wang XH 1,2,3; Jin LF 1,4; Ji SH 1,2; Guo XY 1,2,3; Chen HW 1,2; Ji WZ*1,3,5; wji@mail.kiz.ac.cn
	2011
发表期刊	CELL BIOLOGY INTERNATIONAL
卷号	35 期号:8 页码:775-781
合作性质	其它
摘要	rES (rhesus monkey embryonic stem) cells have similar characteristics to human ES (embryonic stem) cells, and might be useful as a substitute model for preclinical research. Before their clinical application, it is critical to understand the roles of factors that control the differentiation of ES cells into hepatocytes. Here, we analysed the effect of collagen gels on rES cells differentiation into hepatocytes by stepwise protocols. About 80% of DE (definitive endoderm) cells were generated from rES cells after being treated with activin A. The DE cells were then plated on to collagen gels or type I collagen-coated wells with growth factors to induce hepatocyte differentiation. In type I collagen systems, characteristics of immature hepatocytes were observed, including the expression of immature hepatic genes and the generation of 15 +/- 3% AFP (alpha fetoprotein)/CK (cytokeratin)18 double-positive cells. In collagen gel culture, differentiated cells exhibited typical hepatocyte morphology and expressed adult liver-specific genes. The mRNA expression of AFP (immature hepatic gene) was detected at day 11 but decreased at day 18. In contrast, mRNA expression of albumin (mature hepatic gene) was detected at day 11 and increased at day 18. Compared with type I collagen systems, significantly higher AFP/CK18 double-positive cells (68 +/- 7%) were produced in collagen gel culture. Furthermore, some differentiated cells acquired the hepatocytic function of glycogen storage. However, only immature hepatic genes were observed in collagen gel systems if growth factors were absent. Thus, collagen gels combined with hepatocyte-inducing growth factors efficiently promoted differentiation of hepatocytes from rES.
关键词	Collagen Gel Embryonic Stem Cell Growth Factor Hepatocyte Rhesus Monkey
资助者	This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW- R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Develop- ment Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]; and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911]. ; This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW- R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Develop- ment Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]; and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911]. ; This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW- R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Develop- ment Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]; and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911]. ; This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW- R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Develop- ment Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]; and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911].
收录类别	SCI
语种	英语
资助者	This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW- R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Develop- ment Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]; and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911]. ; This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW- R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Develop- ment Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]; and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911]. ; This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW- R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Develop- ment Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]; and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911]. ; This work was supported by the Chinese Academy of Sciences [grant numbers KSCX1-05-02, KSCX1-YW-22-03 and KSCX2-YW- R-47]; the Major State Basic Development Program [grant number 2007CB947701]; the Social Science and Technology Develop- ment Program of Yunnan Province [grant number 2007GH]; the National Science and Technology Major Project [grant number 2009ZX09501-028]; and the Zhejiang Provincial Natural Science Foundation of China [grant number Y2110911].
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6756
专题	生殖与发育生物学中国科学院昆明灵长类研究中心
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, People’s Republic of China 2.Graduate School, Chinese Academy of Sciences, Beijing, People’s Republic of China 3.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming, Yunnan, People’s Republic of China 4.College of Life Science, Shaoxing University, Shaoxing, Zhejiang, People’s Republic of China 5.Kunming Biomed International, Kunming, Yunnan 650500, People’s Republic of China
推荐引用方式 GB/T 7714	Wang XH,Jin LF,Ji SH,et al. Hepatocytic differentiation of rhesus monkey embryonic stem cells promoted by collagen gels and growth factors[J]. CELL BIOLOGY INTERNATIONAL,2011,35(8):775-781.
APA	Wang XH.,Jin LF.,Ji SH.,Guo XY.,Chen HW.,...&wji@mail.kiz.ac.cn.(2011).Hepatocytic differentiation of rhesus monkey embryonic stem cells promoted by collagen gels and growth factors.CELL BIOLOGY INTERNATIONAL,35(8),775-781.
MLA	Wang XH,et al."Hepatocytic differentiation of rhesus monkey embryonic stem cells promoted by collagen gels and growth factors".CELL BIOLOGY INTERNATIONAL 35.8(2011):775-781.

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