Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis

	Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis
	Liu R 1; Mu LX 2; Liu H 2; Wei L 1; Yan TH 3; Chen M 4; Zhang KY 1; Li JX 2; You DW 2; Lai R*1,2; rlai72@njau.edu.cn
	2011
发表期刊	JOURNAL OF PEPTIDE SCIENCE
卷号	17 期号:9 页码:627-631
合作性质	其它
摘要	Two antimicrobial peptides (piceain 1 and 2) derived from sequences encoded Picea sitchensis are identified. Their amino acid sequences are KSLRPRCWIKIKFRCKSLKF and RPRCWIKIKFRCKSLKF, respectively. One intra-molecular disulfide bridge is formed by these two half-cysteines in both piceain 1 and 2. Antimicrobial activities of synthesized piceains against several kinds of microorganisms were tested. They showed antimicrobial activities against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and fungus Candida albicans but little antimicrobial activity against Bacillus subtilis. The results of nematicidal test showed they exerted strong nematicidal activities against Caenorhabditis elegans, following exposure for 5 h at concentrations as low as 10 mu g/ml. They had weak hemolytic abilities against human and rabbit red cells. At the concentration of 250 mu g/ml, they induced red cell hemolysis of less than 5%. Circular dichroism spectra of the two antimicrobial peptides were investigated in several solutions. Their main secondary structure components are beta-sheet and random. The current work provides a novel family of antimicrobial and nematicidal peptides with unique disulfided loop containing nine amino acid residues.
关键词	Picea Sitchensis Antimicrobial And Nematicidal Peptides Innate Immunity
资助者	This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B).
收录类别	SCI
语种	英语
资助者	This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6784
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai72@njau.edu.cn
作者单位	1.Laboratory of Microbiological Engineering of Agricultural Environment, MinistryofAgriculture,LifeSciencesCollegeofNanjingAgriculturalUniversity, Nanjing,Jiangsu,210095,Chin 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming650223, Yunnan, China 3.DepartmentofPhysiology,ChinaPharmaceuticalUniversity,Nanjing,Jiangsu, 210095,China 4.The Department of Nephrology, Teaching Hospital of Chengdu University of TraditionalChineseMedical,Chengdu610072,China
推荐引用方式 GB/T 7714	Liu R,Mu LX,Liu H,et al. Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis[J]. JOURNAL OF PEPTIDE SCIENCE,2011,17(9):627-631.
APA	Liu R.,Mu LX.,Liu H.,Wei L.,Yan TH.,...&rlai72@njau.edu.cn.(2011).Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis.JOURNAL OF PEPTIDE SCIENCE,17(9),627-631.
MLA	Liu R,et al."Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis".JOURNAL OF PEPTIDE SCIENCE 17.9(2011):627-631.

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