A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies

	A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies
	An S 1,3,4; Ma D 1,4; Wei JF 2; Yang X 1,3; Yang HW 2; Yang H 1,4; Xu X 1; He S2; Lai R1; shoahenghe@hotmail.com ; rlai@mail.kiz.ac.cn
	2011
发表期刊	ALLERGY
卷号	66 期号:11 页码:1420-1427
合作性质	其它
摘要	Background: Horsefly sting causes allergic reactions in human body. However, our knowledge on horsefly allergens remains poor. Objectives: To identify the novel horsefly allergens and characterize their properties. Methods: A native allergen protein Tab y 1 (apyrase) was purified from the salivary glands of the horsefly Tabanus yao Macquart by gel filtration and ion exchange chromatography. Its sequence was determined by Edman degradation and cDNA cloning. Its allergenicity was assessed by immunoblotting for specific IgE, basophil activation test, skin prick test (SPT), and competitive enzyme-linked immunosorbent assay (ELISA). Results: Tab y 1 showed a single diffusion band of 70 kDa on SDS-PAGE. Seventy percent (7/10) of patients with horsefly allergy tested positive to Tab y 1 in SPT; sera from 81% (30/37) of patients reacted to Tab y 1 on western blots. Purified Tab y 1 reduced approximately 42% sera IgE reactivity to horsefly salivary gland extract on a competitive ELISA. Tab y 1 upregulated the expression of CD63 and CCR3 on passively sensitized basophils by up to approximately 4.9-fold. Tab y 1 also showed enzymatic activity to hydrolyze ATP and ADP, and potent antiplatelet aggregation and antithrombotic activities. Conclusion: The current work identified a novel major allergen of horsefly, Tab y 1, with antiplatelet aggregation and antithrombotic activities, which implicates Tab y 1 in helping horseflies suck host blood, meanwhile causing allergy in their human hosts.
关键词	Apyrase Basophil Cd63 Horsefly Allergen Insect Allergy
资助者	The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B). ; The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B). ; The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B). ; The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B).
收录类别	SCI
语种	英语
资助者	The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B). ; The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B). ; The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B). ; The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6826
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	shoahenghe@hotmail.com; rlai@mail.kiz.ac.cn
作者单位	1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 2.Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing 3.School of Life Sciences, University of Science and Technology of China, Hefe 4.Graduate School of the Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	An S,Ma D,Wei JF,et al. A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies[J]. ALLERGY,2011,66(11):1420-1427.
APA	An S.,Ma D.,Wei JF.,Yang X.,Yang HW.,...&rlai@mail.kiz.ac.cn.(2011).A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies.ALLERGY,66(11),1420-1427.
MLA	An S,et al."A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies".ALLERGY 66.11(2011):1420-1427.

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