Molecular phylogeography of Jerdon's pitviper (Protobothrops jerdonii): importance of the uplift of the Tibetan plateau

	Molecular phylogeography of Jerdon's pitviper (Protobothrops jerdonii): importance of the uplift of the Tibetan plateau
	Guo P*1,2; Liu Q 1; Li C 1; Chen X 3; Jiang K 4; Wang YZ 2; Malhotra A 5; ybguop@163.com
	2011
发表期刊	JOURNAL OF BIOGEOGRAPHY
卷号	38 期号:12 页码:2326-2336
合作性质	其它
摘要	Aim To investigate the population history and demographics of Jerdons pitviper, Protobothrops jerdonii, and elucidate how the unique physical conditions and heterogeneous mountain environments resulting from the uplift of the Tibetan Plateau shaped the genetic diversity and evolutionary history of the species. Location China and Vietnam. Methods We sequenced and analysed a total of 1752 base pairs from two mitochondrial genes, cytochrome b (cyt b) and NADH dehydrogenase subunit (ND4), for 81 specimens sampled from 27 localities across the species' range, and a total of 464 base pairs from two nuclear genes for 28 representative samples from all mitochondrial DNA lineages. Based on these data, we constructed the genealogical relationships and estimated the divergence times of the mitochondrial DNA clades. Results The mitochondrial DNA results revealed the existence of five distinct, strongly supported and geographically structured DNA lineages within populations of P. jerdonii that are paraphyletic with respect to Protobothrops xiangchengensis. Estimation of divergence dates suggested that P. jerdonii possibly evolved in the western Hengduan Mountains region c. 6.6 Ma in the late Miocene. Nuclear DNA data did not provide sufficient resolution to distinguish the mitochondrial DNA lineages. Main conclusions Based on the present-day distribution and intraspecific genealogy, the evolutionary history of P. jerdonii can be explained by a pattern of dispersal followed by vicariance. All lines of evidence suggest that historical biogeographical factors, particularly the north-south orientation of the higher mountains, as well as low-elevation areas in western China, had the greatest influence on the population structure, lineage formation and species distribution of this snake. However, highly heterogeneous habitats and glacial cycles appear to have affected patterns of intraspecific differentiation. While our mitochondrial data provide evidence for clear phylogeographical structure, our small sampling of nuclear genes does not, suggesting that nuclear markers may not have had sufficient time to coalesce to match patterns observed in the mitochondrial data
关键词	Asia China Dispersal High-elevation Biogeography Highland Fauna
资助者	This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 30670236, NSFC 30970334, NSFC 31071892), the Program for New Century Excellent Talents in University, the Ministry of Education of the People’s Republic of China (NCET-08-0908) and Sichuan Youth Sciences and Technology Foundation (08ZQ026-006). P.G. and A.M. thank the Royal Society for additional funding (International Project Grant IJP2006/R1). ; This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 30670236, NSFC 30970334, NSFC 31071892), the Program for New Century Excellent Talents in University, the Ministry of Education of the People’s Republic of China (NCET-08-0908) and Sichuan Youth Sciences and Technology Foundation (08ZQ026-006). P.G. and A.M. thank the Royal Society for additional funding (International Project Grant IJP2006/R1). ; This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 30670236, NSFC 30970334, NSFC 31071892), the Program for New Century Excellent Talents in University, the Ministry of Education of the People’s Republic of China (NCET-08-0908) and Sichuan Youth Sciences and Technology Foundation (08ZQ026-006). P.G. and A.M. thank the Royal Society for additional funding (International Project Grant IJP2006/R1). ; This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 30670236, NSFC 30970334, NSFC 31071892), the Program for New Century Excellent Talents in University, the Ministry of Education of the People’s Republic of China (NCET-08-0908) and Sichuan Youth Sciences and Technology Foundation (08ZQ026-006). P.G. and A.M. thank the Royal Society for additional funding (International Project Grant IJP2006/R1).
收录类别	SCI
语种	英语
资助者	This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 30670236, NSFC 30970334, NSFC 31071892), the Program for New Century Excellent Talents in University, the Ministry of Education of the People’s Republic of China (NCET-08-0908) and Sichuan Youth Sciences and Technology Foundation (08ZQ026-006). P.G. and A.M. thank the Royal Society for additional funding (International Project Grant IJP2006/R1). ; This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 30670236, NSFC 30970334, NSFC 31071892), the Program for New Century Excellent Talents in University, the Ministry of Education of the People’s Republic of China (NCET-08-0908) and Sichuan Youth Sciences and Technology Foundation (08ZQ026-006). P.G. and A.M. thank the Royal Society for additional funding (International Project Grant IJP2006/R1). ; This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 30670236, NSFC 30970334, NSFC 31071892), the Program for New Century Excellent Talents in University, the Ministry of Education of the People’s Republic of China (NCET-08-0908) and Sichuan Youth Sciences and Technology Foundation (08ZQ026-006). P.G. and A.M. thank the Royal Society for additional funding (International Project Grant IJP2006/R1). ; This project is partly supported by grants from the National Natural Science Foundation of China (NSFC 30670236, NSFC 30970334, NSFC 31071892), the Program for New Century Excellent Talents in University, the Ministry of Education of the People’s Republic of China (NCET-08-0908) and Sichuan Youth Sciences and Technology Foundation (08ZQ026-006). P.G. and A.M. thank the Royal Society for additional funding (International Project Grant IJP2006/R1).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6871
专题	科研部门_两栖爬行类多样性与进化（车静）遗传资源与进化国家重点实验室
通讯作者	ybguop@163.com
作者单位	1.College of Life Sciences and Food Engineering, Yibin University, Yibin 644007, Sichuan, China 2.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610064, China 3.Department of Biology, 2800 Victory Boulevard, College of Staten Island — City University of New York, Staten Island, NY 10314, USA 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 5.School of Biological Sciences, College of Natural Sciences, Bangor University, Gwynedd LL57 2UW, UK
推荐引用方式 GB/T 7714	Guo P*,Liu Q,Li C,et al. Molecular phylogeography of Jerdon's pitviper (Protobothrops jerdonii): importance of the uplift of the Tibetan plateau[J]. JOURNAL OF BIOGEOGRAPHY,2011,38(12):2326-2336.
APA	Guo P*.,Liu Q.,Li C.,Chen X.,Jiang K.,...&ybguop@163.com.(2011).Molecular phylogeography of Jerdon's pitviper (Protobothrops jerdonii): importance of the uplift of the Tibetan plateau.JOURNAL OF BIOGEOGRAPHY,38(12),2326-2336.
MLA	Guo P*,et al."Molecular phylogeography of Jerdon's pitviper (Protobothrops jerdonii): importance of the uplift of the Tibetan plateau".JOURNAL OF BIOGEOGRAPHY 38.12(2011):2326-2336.

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