Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction

KIZ OpenIR > 分子病理学

	Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction
	Chang H 1,2; Zhang C 1,2; Cao Y*1; caoy@mail.kiz.ac.cn
	2012
发表期刊	HISTOCHEMISTRY AND CELL BIOLOGY
卷号	137 期号:3 页码:319-327
合作性质	其它
摘要	Symplekin is multifunctional protein localized to both the tight junction and the nucleus with known roles in mRNA polyadenylation, proliferation, differentiation and tumorigenesis. Functions of symplekin at tight junctions have not been systematically investigated. In this study, increased expression of symplekin was observed during the formation of tight junctions in cultured HT-29 and HepG2 epithelial cells. Repression of symplekin by RNAi increased the permeability of epithelial monolayers, disrupted cellular polarity, and decreased the expression of the tight junction protein ZO-1. Moreover, symplekin was co-localized with ZO-1 at tight junctions and co-immunoprecipitated with ZO-1, indicating that ZO-1 and symplekin form complexes. In conclusion, symplekin expression regulates the assembly of tight junctions, thus helps to maintain the integrity of the epithelial monolayer and cellular polarity
关键词	Symplekin Tight Junction Permeability Polarity Zo-1
资助者	This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao). ; This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao). ; This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao). ; This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao).
收录类别	SCI
语种	英语
资助者	This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao). ; This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao). ; This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao). ; This study was supported by National Natural Science Foundation of China (No. 30800407) and Human Talents Pro- gram of Chinese Academy of Sciences (Grant Cao).
WOS记录号	WOS:000300326100005
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6875
专题	分子病理学科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	caoy@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanism of CAS and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan 650223, Peoples’ Republic of China 2.Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Chang H,Zhang C,Cao Y*,et al. Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2012,137(3):319-327.
APA	Chang H,Zhang C,Cao Y*,&caoy@mail.kiz.ac.cn.(2012).Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction.HISTOCHEMISTRY AND CELL BIOLOGY,137(3),319-327.
MLA	Chang H,et al."Expression and distribution of symplekin regulates the assembly and function of the epithelial tight junction".HISTOCHEMISTRY AND CELL BIOLOGY 137.3(2012):319-327.

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