Immunogenicity of Lactobacillus-expressing VP2 and VP3 of the infectious pancreatic necrosis virus (IPNV) in rainbow trout

	Immunogenicity of Lactobacillus-expressing VP2 and VP3 of the infectious pancreatic necrosis virus (IPNV) in rainbow trout
	Liu M 1; Zhao LL 1; Ge JW 1; Qiao XY 1; Li YJ1; Liu DQ2; yijingli@yahoo.com ; liudiqiu@mail.kiz.ac.cn
	2012
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	32 期号:1 页码:196-203
合作性质	其它
摘要	Infectious pancreatic necrosis virus (IPNV) infects salmonid fish with high mortality and causes serious economic losses to salmonid aquaculture. Lactobacillus strains have a number of properties that make them attractive candidates as delivery vehicles for the presentation to the mucosa of compounds with pharmaceutical interest, in particular vaccines. Here, Lactobacilli/Escherichia coli shuttle vector pPG1 (surface-displayed) or pPG2 (secretory) with the capsid VP2 gene inserted was transformed into Lactobacillus casei to yield two recombinant strains: Lc:PG1-vP2 and Lc:PG2-VP2, respectively. Rainbow trout immunized respectively with Lc:PG1-VP2, Lc:PG2-VP2, Lc:PG1-VP3 and Lc:PG2-VP3 elicited anti-IPNV immune responses (serum IgM) via oral route. Statistical results of serum IgM titer with neutralizing activity showed that immunogenicity of Lc:PG2-VP2 was more powerful than that of Lc:PG1-VP2 (P < 0.001), Lc:PG1-VP3 (P < 0.001) and Lc:PG2-VP3 (P < 0.001), which was confirmed by viral loads reduction analyzed by real-time RT-PCR in orally immunized rainbow trout after virus challenge. Comparing with negative control, rainbow trout orally dosed with Lc:PG2-VP2 resulted in similar to 46-fold reduction in virus load on days 10 post viral challenge as well as Lc:PG1-VP2(similar to 20-fold), Lc:PG2-VP3(similar to 6-fold) and Lc:PG1-VP3(similar to 3-fold). Taken together, Lc:PG2-VP2 exhibited a more appropriate candidate as live bacteria vaccine against IPNV infection in rainbow trout.
关键词	Infectious Pancreatic Necrosis Virus (Ipnv) Vp2 And Vp3 Lactobacillus Oral Vaccine Rainbow Trout
资助者	This study was supported by Natural Science Foundation of Heilongjiang province (C200837). ; This study was supported by Natural Science Foundation of Heilongjiang province (C200837). ; This study was supported by Natural Science Foundation of Heilongjiang province (C200837). ; This study was supported by Natural Science Foundation of Heilongjiang province (C200837).
收录类别	SCI
语种	英语
资助者	This study was supported by Natural Science Foundation of Heilongjiang province (C200837). ; This study was supported by Natural Science Foundation of Heilongjiang province (C200837). ; This study was supported by Natural Science Foundation of Heilongjiang province (C200837). ; This study was supported by Natural Science Foundation of Heilongjiang province (C200837).
WOS记录号	WOS:000299979100023
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6894
专题	科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	yijingli@yahoo.com; liudiqiu@mail.kiz.ac.cn
作者单位	1.Veterinary Microbiology Department, Veterinary Medicine College, Northeast Agricultural University, Harbin 150030, PR China 2.Key Laboratory of Animal Models and Human Diesease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China
推荐引用方式 GB/T 7714	Liu M,Zhao LL,Ge JW,et al. Immunogenicity of Lactobacillus-expressing VP2 and VP3 of the infectious pancreatic necrosis virus (IPNV) in rainbow trout[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(1):196-203.
APA	Liu M.,Zhao LL.,Ge JW.,Qiao XY.,Li YJ*.,...&liudiqiu@mail.kiz.ac.cn.(2012).Immunogenicity of Lactobacillus-expressing VP2 and VP3 of the infectious pancreatic necrosis virus (IPNV) in rainbow trout.FISH & SHELLFISH IMMUNOLOGY,32(1),196-203.
MLA	Liu M,et al."Immunogenicity of Lactobacillus-expressing VP2 and VP3 of the infectious pancreatic necrosis virus (IPNV) in rainbow trout".FISH & SHELLFISH IMMUNOLOGY 32.1(2012):196-203.

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