Extraction, PCR amplification, and sequencing of mitochondrial DNA from scent mark and feces in the giant panda

	Extraction, PCR amplification, and sequencing of mitochondrial DNA from scent mark and feces in the giant panda
	Ding B 1; Zhang YP1 ; Ryder OA*2
	1998
发表期刊	ZOO BIOLOGY
ISSN	0733-3188
卷号	17 期号:6 页码:499-504
合作性质	其它
摘要	To expand the feasibility of applying simple, efficient, non-invasive DNA preparation methods using samples that can be obtained from giant pandas living in the wild, we investigated the use of scent markings and fecal samples. Giant panda-specific oligonucleotide primers were used to amplify a portion of the mitochondrial DNA control region as well as a portion of the mitochondrial DNA cytochrome b gene and tRNA(Thr) gene region. A 196 base pair (bp) fragment in the control region and a 449 bp fragment in the cytochrome b gene and tRNA(Thr) gene were successfully amplified. Sequencing of polymerase chain reaction (PCR) products demonstrated that the two fragments are giant panda sequences. Furthermore, under simulated field conditions we found that DNA can be extracted from fecal samples aged as long as 3 months. Our results suggest that the scent mark and fecal samples are simple, efficient, and easily prepared DNA sources.
关键词	Genetic Monitoring Non-invasive Conservation Genetics
资助者	This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust.
URL	查看原文
收录类别	SCI
语种	英语
资助者	This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust. ; This work was supported by the Zoological Society of San Diego and the Alice C. Tyler Perpetual Trust.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3881
专题	科研部门_分子进化与基因组多样性（张亚平）细胞与分子进化开放实验室
作者单位	1.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, China 2.Center for Reproduction of Endangered Species, Zoological Society of San Diego, San Diego, California
推荐引用方式 GB/T 7714	Ding B,Zhang YP,Ryder OA*. Extraction, PCR amplification, and sequencing of mitochondrial DNA from scent mark and feces in the giant panda[J]. ZOO BIOLOGY,1998,17(6):499-504.
APA	Ding B,Zhang YP,&Ryder OA*.(1998).Extraction, PCR amplification, and sequencing of mitochondrial DNA from scent mark and feces in the giant panda.ZOO BIOLOGY,17(6),499-504.
MLA	Ding B,et al."Extraction, PCR amplification, and sequencing of mitochondrial DNA from scent mark and feces in the giant panda".ZOO BIOLOGY 17.6(1998):499-504.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
199817499.pdf（58KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文